15 Piv txwv ntawm Centrifugation - Phau Ntawv Qhia Txog Kev

Daim Duab: Centrifugation| Separation Methods | Physics

Zoo Siab

Hom centrifugation
Piv txwv ntawm centrifugation

Cov centrifugation Nws yog ib txoj hauv kev cais cov khoom sib txawv los ntawm cov kua sib txawv hauv qhov sib xyaw, tsuav yog cov qub tsis tuaj yeem siv, siv lub zog quab yuam lossis lub zog centrifugal.

Txog qhov no, ib qho cuab yeej hu ua centrifuge lossis centrifuge feem ntau siv, uas tig qhov sib tov sib xyaw ntawm qhov ruaj khov thiab txiav txim siab.

Raws li nws lub npe qhia (centrifuge: khiav tawm ntawm qhov chaw), qhov kev quab yuam no thawb lub densest Cheebtsam tawm ntawm lub axis ntawm kev sib hloov, tawm hauv qhov tsawg dua qhov uas nyob hauv nruab nrab nws tus kheej. Nws yog qhov tsis sib haum rau lub zog centripetal.

Saib kuj: Chromatography

Hom centrifugation

Sib txawv. Raws li qhov sib txawv ntawm qhov ceev ntawm cov tshuaj, nws yog qhov yooj yim tab sis cov txheej txheem tsis muaj tseeb.
Isopychnic. Cov txheej txheem no tau siv, piv txwv li, kom cais cov khoom sib txawv ntawm qhov loj me me tab sis muaj qhov sib txawv sib txawv.
Zonal. Qhov sib txawv ntawm tus nqi qis ntawm cov tshuaj (vim lawv qhov sib txawv sib txawv) tau siv los cais lawv nyob rau lub sijhawm muab centrifugation.
Ultracentrifugation. Nws lub zog tso cai rau kev sib cais ntawm cov molecules thiab cov tshuaj subcellular.

Piv txwv ntawm centrifugation

Lub tshuab ntxhua khaub ncaws. Cov khoom siv no siv lub zog centrifugal cais cov khaub ncaws (khoom) los ntawm dej (kua) raws li lawv qhov hnyav. Tias yog vim li cas cov khaub ncaws feem ntau yuav luag qhuav thaum tshem tawm sab hauv.
Kev lag luam mis nyuj. Cov mis nyuj tau muab tso rau hauv ib nrab kom faib nws cov dej thiab lipid cov ntsiab lus, txij li tom kawg tau siv los ua butter, lossis cov mis nyuj skimmed los ntawm qhov seem.
Cov tsheb hauv qhov nkhaus. Thaum tsav tsheb nrawm hla txoj kab nkhaus hauv txoj kev, peb feem ntau hnov lub zog rub peb tawm ntawm txoj kev, kom deb ntawm txoj kab nkhaus. Qhov ntawd yog lub zog centrifugal.
Tau txais cov enzymes. Hauv kev kho mob thiab tshuaj lag luam, feem ntau siv cov centrifugation kom tau txais qee cov enzymes los ntawm cov cell tshwj xeeb uas tsim lawv.
DNA sib cais. Isopycnic centrifugation feem ntau siv hauv cov chaw soj ntsuam caj ces kom cais cov cell cell DNA thiab tso cai rau nws kawm ntxiv thiab siv.
Khoom noj rau celiacs. Thaum nws los cais cov protein los ntawm gluten los ntawm cov khoom noj uas muaj nws, cov txheej txheem centrifugation yog qhov tseem ceeb. Nws tau ua tiav ntawm cov hmoov txhuv nplej siab muab tshuaj, nws cov ntsiab lus gluten nce mus txog 8%, thiab tau txo qis qis dua 2% hauv kev xaiv ua ntu zus centrifugation.
Kev kuaj ntshav Lub centrifuge yog siv los cais cov ntsiab lus ntawm cov ntshav, xws li ntshav thiab lwm yam uas feem ntau sib xyaw hauv nws.
Acceleration ntawm sedimentation. Hauv ntau yam kev lag luam khoom noj khoom haus, xws li brewing lossis cereals, centrifugation ua kom nrawm dua cov txheej txheem sedimentation uas tshwm sim ntawm lub ntiajteb txawj nqus tsim, txo lub sijhawm tos ntawm cov khoom siv raw.
Ntxuav cov yas. Hauv kev lag luam roj hmab, nws yog qhov tsim nyog los ntxuav cov tshuaj, uas nws qhov tshwj xeeb yog qhov ua tau zoo rau kev ua raws li lwm cov khoom, thiab qhov no tau ua los ntawm kev ua kom centrifugation, muab qhov ntom ntom tsawg.
Qhuav cov khib nyiab. Lwm daim ntawv thov kev lag luam ntawm cov centrifuge yog kom qhuav ntawm cov muaju lossis lwm yam khoom siv uas nws cov khoom tsim nrog dej. Raws li nws tig, cov dej sib cais los ntawm cov khib nyiab thiab muab pov tseg, tawm hauv cov khoom xav tau yam tsis muaj kua.
Kev kho dej phwj tuaj. Qhov centrifugation ntawm cov dej ua paug tso cai rho tawm ntawm cov tshuaj sib tov sab hauv, tsis yog tsuas yog cov khib nyiab, tab sis tseem muaj roj, rog thiab lwm yam tsis xav tau uas, ib zaug centrifuged, tuaj yeem muab pov tseg.
Chaw ua si lom zem. Ntau qhov kev lom zem ua si caij tsheb siv lub zog centrifugal los tsim lub tshuab nqus tsev rau lawv cov neeg caij tsheb, uas tau nrawm nrawm rau ntawm lub axis ruaj khov, nruj nreem txuas rau lub rooj zaum uas tiv thaiv lawv kom tsis txhob raug ntiab tawm ntawm txoj kab kev sib hloov.
Pirouette cov neeg caij tsheb kauj vab. Tus neeg caij tsheb kauj vab nyob rau hauv ib puag ncig yog qhov qub ntawm cov circus, leej twg tuaj yeem tsav hla lub ru tsev ntawm tus kheej tiv thaiv lub ntiajteb txawj nqus. Qhov no muaj peev xwm ua nws tom qab tau ua ntau yam tig rau tib kab rov tav, ua kom nrawm dua thiab xa mus rau lub zog centrifugal uas ua raws nws mus rau sab hauv ntawm tus kheej. Thaum kawg qhov kev quab yuam no yuav zoo heev uas nws yuav tuaj yeem ua lub zog txav mus los thiab tiv thaiv lub ntiajteb txawj nqus.
Kev xav ntawm txoj kev tsheb ciav hlau. Txhawm rau tiv thaiv lub zog centrifugal, cov tsheb ciav hlau feem ntau qaij mus rau hauv txoj kab nkhaus, ua rau muaj kev tiv thaiv kom nws tsis swb rau lub zog uas thawb nws sab nraud thiab tsis poob.
Kev txhais lus hauv av. Qhov laj thawj uas lub zog nqus ntawm lub Hnub tsis thawb peb mus rau hauv nws kuj yog vim lub zog centrifugal uas, thaum tig rau ntawm lub axis ntawm lub hnub huab tais, thawb nws sab nraud, tawm tsam thiab ua kom lub ntiajteb txawj nqus nyiam.